Chromatography

Chromatography is separative technique whereby the components of a mixture may be separated by allowing the sample (analyte) to be transported through a packed bed of material (the stationary phase) by a fluid ,obile phase. if the individual components in the mixture move through the packed bed at the different rates then separation will occur, the degree of separation depending on the different in the rates of migration. the International of Pure and Applied Chemistry (IUPAC) has ddefined chromatography as a method used primarily for the separation of components of a xample, in which the components are distributed between two phases, one of which is stationary while the other moves. the stationary phase may be a solid, or a liquid supported on a solid, or a gel.The stationary phase may be packed in a column, spread as a layer, or distributed as a film, etc. In this definition, chromatography bed is used as a general term to denote any of the different forms in which the stationary phase may be used. the mobile phase may be gaseous or liquid.